A complete list of all those serving as Rector at Chapel of the Cross.
- 1842-1847: William Mercer Green
- 1848-1849: Aaron F. Olmstead
- 1849-1851: Thomas F. Davis (Deacon)
- 1851-1853: Fordyce Mitchell Hubbard
- 1853-1855: John Thomas Wheat
- 1856-1858: Henry T. Lee
- 1858-1859: John Thomas Wheat
- 1861-1865: Francis William Hillard
- 1865-1868: Fordyce Mitchell Hubbard
- 1868-1878: No rectors served Chapel of the Cross while the University was closed for reconstruction.
- 1878-1881: Joseph Blount Cheshire
- 1881-1883: Edmund Noah Joyner
- 1883-1884: John Huske
- 1885: Malcolm Douglas
- 1886-1887: William Meade Clark
- 1889-1890: Augustine Prentiss,
- 1890-1891: Edward Manning Gushee
- 1891-1894: Frederick Towers,
- 1895-1896: Louis Hector Shubert
- 1896-1898: Thomas Edward Winecoff
- 1898-1908: William Hopkins Meade
- 1908-1911: Richard Wallace Hogue
- 1911-1916: Homer Worthington Starr
- 1917-1920: Richard Maynard Marshall
- 1921-1944: Alfred Stratton Lawrence
- 1945-1959: David Watt Yates
- 1960-1970: Thomas Robinson Thrasher
- 1971-1984: Peter James Lee
- 1985-2015: Stephen John Elkins-Williams
- 2016-Present: Elizabeth Marie Melchionna